FAS Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

FAS

Synonyms

ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.3-2 μg/mL in the presence of recombinant human Fas ligand.

Function

Met1-Glu173

Molecular Weight

The recombinant human Fas/Fc chimera is a disulfide-linked homodimeric protein generated after removal of the signal peptide. The reduced monomer consists of 386 amino acids and has a predicted molecular mass of 43.4 kDa. In SDS-PAGE under reducing conditions, the monomer migrates as an approximately 55-60 kDa protein due to glycosylation.