FAS Protein (C-human IgG1-Fc, Cynomolgus)

1. Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.5-3 μg/mL in the presence of 200 ng/mL recombinant human Fas ligand. 2. Measured by its binding ability in a functional ELISA. Immobilized human His-FASLG (Cat:10244-H07Y) at 10 μg/ml (100 μl/well) can bind Cynomolgus FAS-Fc. The EC50 of Cynomolgus FAS-Fc is 0.06-0.14 μg/ml.

The recombinant cynomolgus FAS is a disulfide-linked homodimer. The reduced monomer comprises 389 amino acids and has a calculated molecular mass of 43.8 KDa.The apparent molecular mass of the protein is approximately 47-57 KDa in SDS-PAGE.