FAP Protein (N-human IgG1-Fc, Human)

Product Specifications

Abbreviation

FAP

Synonyms

DPPIV, FAPA, FAPalpha, SIMP

Host

HEK 293

Tag

N-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Recombinant Human FAP Protein (ECD, Fc Tag) (Cat: 10464-H01H) at 1 μg/mL (100 μL/well) can bind Monoclonal anti-FAP antibody, human IgG1, the EC50 is 0.6-2.5 ng/mL (QC tested) . 2.Loaded Anti-FAP Antibody, Human IgG1 on AR2G Biosensor, can bind Recombinant Human FAP Protein (ECD, hFc Tag) (Cat. No. 10464-H01H) with an affinity constant of 0.134 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Leu26-Asp760

Molecular Weight

The recombinant Human FAP comprises 972 amino acids and has a predicted molecular mass of 111.7 kDa. It migrates as approximately 120.6 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 246.0 kDa verified by SEC-MALS (Routinely tested) .