F3 Protein (C-His, Mouse)
Product Specifications
Abbreviation
F3
Synonyms
CD142, Cf-3, Cf3, TF
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
≥ 98 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
Bioactivity
Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC (R&D Systems, Catalog# ES011) . The AC50 is < 5 μg/ml .
Function
Met1-Glu251
Molecular Weight
The secreted recombinant mouse F3 consists of 234 amino acids and has a calculated molecular mass of 26.8 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 40-45 kDa in SDS-PAGE under reducing conditions.
Species
Mouse
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