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F3 Protein (C-His, Mouse)

Product Specifications

Abbreviation

Synonyms

CD142, Cf-3, Cf3, TF

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 98 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC (R&D Systems, Catalog# ES011) . The AC50 is < 5 μg/ml .

Function

Met1-Glu251

Molecular Weight

The secreted recombinant mouse F3 consists of 234 amino acids and has a calculated molecular mass of 26.8 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 40-45 kDa in SDS-PAGE under reducing conditions.