F11 Protein (C-His, Human)

Product Specifications

Abbreviation

F11

Synonyms

FXI, PTA

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 85 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC) . The specific activity is >100 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

Function

Met1-Val625

Molecular Weight

The secreted recombinant human F11 consists of 618 amino acids with the predicted molecular mass of 69.5 kDa. As a result of glycosylation, rhCTSS migrates as an approximately 75-80 kDa band in SDS-PAGE under reducing conditions.