ERBB2 Protein (C-human IgG1-Fc, Cynomolgus)

Product Specifications

Abbreviation

ERBB2

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 85 % as determined by SDS-PAGE.

Bioactivity

1.Immobilized Her2/ERBB2 Protein, Cynomolgus, Recombinant (ECD, hFc Tag) (Cat:90295-C02H) at 2 μg/ml (100 μl/well) can bind Trastuzumab derived Anti-Erbb2 Antibody, Human IgG1, the EC50 is 15-70 ng/mL (QC tested) . 2.Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.3-1.8 μg/mL in the presence of 0.6 μg/mL Anti-ErbB2/Her2 Monoclonal Antibody (Routinely tested) . 3.Immobilized Recombinant Cynomolgus HER2 / ErbB2 Protein (ECD, Fc Tag) (Cat: 90295-C02H) at 1 μg/mL (100 μL/well) can bind Anti-HER2 (Research Grade Pertuzu*mab Biosimilar), the EC50 is 13.7 ng/mL (Routinely tested) .