ERBB2 Protein (C-His, Rhesus)

1.Measured by its binding ability in a functional ELISA. Immobilized Rhesus Her2/ERBB2 His (Cat:90020-K08H) at 2 μg/ml (100 μl/well) can bind Herceptin, the EC50 of Herceptin is 10-50 ng/mL. 2.Loaded Recombinant Rhesus Her2/ERBB2 Protein , His Tag (Cat. No. 90020-K08H) on ProA Biosensor, can bind Herceptin with an affinity constant of 7.91 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant Rhesus ErbB2 consists of 640 amino acids and predicts a molecular mass of 70.7 kDa. As a result of glycosylation, rhesus ErbB2 migrates as an approximately 105 kDa band in SDS-PAGE under reducing conditions.