EPOR Protein (C-human IgG1-Fc, Mouse)

Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.8 μg/mL in the presence of 16 ng/mL Recombinant mouse EPO. 2. Immobilized Recombinant Mouse EPO Receptor / EPOR Protein (Fc Tag) (Cat: 50031-M02B) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse Erythropoietin / EPO Protein (His Tag) (Cat: 51099-M08H), the EC50 is 1.5-5 ng/mL (QC tested) .

The recombinant mouse EPOR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 463 amino acids and has a predicted molecular mass of 51.4 kDa. The apparent molecular mass of the protein is approximately 58.6 kDa in SDS-PAGE under reducing conditions due to glycosylation.