EPOR Protein (C-His, Mouse)

1. Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.5 μg/mL in the presence of 16 ng/mL Recombinant mouse EPO. 2. Measured by its binding ability in a functional ELISA. 3. Immobilized mouse EPOR-His at 10μg/mL (100μL/well) can bind biotinylated mouse EPO-His (Cat:51099-M08H) . The EC50 of biotinylated mouse EPO-His (Cat:51099-M08H) is 34.5-80.6ng/mL.

The recombinant mouse EPOR comprises 236 amino acids with a predicted molecular mass of 26.2 kDa. As a result of glycosylation, the apparent mplecular mass of rmEPOR is approximately 30-35 kDa in SDS-PAGE under reducing conditions.