EphB6 Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

EphB6

Synonyms

EPHB6, HEP

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.

Bioactivity

1. Measured by its binding ability in a functional ELISA. 2. Immobilized recombinant human EphrinB1 at 10 μg/ml (100 μl/well) can bind human EphB6 with a linear range of 0.16-4 μg/ml. 3. Immobilized recombinant human EphrinB2 at 10 μg/ml (100 μl/well) can bind human EphB6 with a linear range of 1.28-32 ng/ml.

Function

Met1-Ser579

Molecular Weight

The recombinant mature human EphB6/Fc chimera is a disulfide linked homodimeric protein. Each monomer consists of 801 amino acids and has a calculated molecular mass of 86.5 kDa. In SDS-PAGE under reducing conditions, rhEphB6/Fc monomer migrates with an apparent molecular mass of approximately 100-110 kDa due to glycosylation.