EPHA5 Protein (C-His, Cynomolgus)

Product Specifications

Abbreviation

EPHA5

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Recombinant Cynomolgus EPHA5 Protein (His Tag) (Cat: 91049-C08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human Ephrin-A3 / EFNA3 / EFL2 Protein (Fc Tag) (Cat: 10188-H02H), the EC50 is 3-12 ng/mL. 2.Loaded Recombinant Human Ephrin A3 Protein, hFc Tag (Cat. No. 10188-H02H) on proA Biosensor, can bind Recombinant Cynomolgus EPHA5 Protein, His Tag (Cat. No. 91049-C08H) with an affinity constant of 78.2 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Ser56-Pro573

Molecular Weight

The recombinant Cynomolgus EPHA5 consists of 529 amino acids and predicts a molecular mass of 59.01 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 76 kDa protein in SDS-PAGE under reducing conditions.