EPHA5 Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

EPHA5

Synonyms

BSK, CEK7, EHK-1, EHK1, EK7, HEK7, TYRO4

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Recombinant Human EPHA5 Protein (ECD, Fc Tag) (Cat: 29795-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human Ephrin-A3 / EFNA3 Protein (His Tag) (Cat: 10188-H08H), the EC50 is 250-750 ng/mL. 2.Loaded Recombinant Human EPHA5 Protein, Fc Tag (Cat. No. 29795-H02H) on proA Biosensor, can bind Recombinant Human Ephrin A3 Protein, His Tag (Cat. No. 10188-H08H) with an affinity constant of 0.446 μM as determined in BLI assay (Sartorius Octet RED384) (QC tested) .

Function

Met1-Pro573

Molecular Weight

The recombinant Human EPHA5 consists of 787 amino acids and predicts a molecular mass of 87.53 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 109.5 kDa protein in SDS-PAGE under reducing conditions.