EGFR Protein (C-His, Mouse)

1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind human EGF-Fc (Cat:10605-H01H), The EC50 of human EGF-Fc (Cat:10605-H01H) is 60-90 ng/mL. 3. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind mouse EGF-Fc (Cat:50482-M01H), The EC50 of mouse EGF-Fc (Cat:50482-M01H) is 70-100 ng/mL. 4.Recombinant Mouse EGF Protein, hFc Tag (Cat. No. 50482-M01H) captured on Protein A chip can bind Recombinant Mouse EGFR Protein, His Tag (Cat. No. 51091-M08H) with an affinity constant of 0.630 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

The secreted recombinant mouse EGFR comprises 634 amino acids and has a calculated molecular mass of 70.8 kDa. As a result of glycosylation, the apparent molecular mass of rmEGFR is approximately 100 kDa in SDS-PAGE under reducing conditions.