EGFR Protein (C-His, Canine)

Product Specifications

Abbreviation

EGFR

Host

Baculovirus-insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized canine EGFR-His at 10 μg/ml (100 μl/well) can bind biotinylated canine His-EGF (Cat:70013-D07E), The EC50 of biotinylated canine His-EGF (Cat:70013-D07E) is 0.16-0.36μg/ml

Function

Met1-Ser618

Molecular Weight

The recombinant canine EGFR comprises 629 amino acids and has a predicted molecular mass of 69.8 kDa. The apparent molecular mass of the protein is approximately 87.2 kDa in SDS-PAGE under reducing conditions.