EGF Protein (N-human IgG1-Fc, Mouse)

Product Specifications

Abbreviation

EGF

Synonyms

Egf

Host

HEK 293

Tag

N-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

1.Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.5-3 ng/mL. 2.Recombinant Mouse EGF Protein, hFc Tag (Cat. No. 50482-M01H) captured on Protein A chip can bind Recombinant Mouse EGFR Protein, His Tag (Cat. No. 51091-M08H) with an affinity constant of 0.630 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

Function

Asn977-Arg1029

Molecular Weight

The recombinant mouse EGF/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 314 amino acids and has a predicted molecular mass of 34.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 44 and 38 kDa.