EGF Protein (N-human IgG1-Fc, Human)

Product Specifications

Abbreviation

EGF

Synonyms

HOMG4, URG

Host

HEK 293

Tag

N-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

1.Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.3-1.5 ng/mL (Routinely tested) . 2.Immobilized Recombinant Human EGF / Epidermal Growth Factor Protein (Fc Tag) (Cat: 10605-H01H) at 2 μg/mL (100 μL/well) can bind Recombinant Human EGFR / HER1 / ErbB1 Protein (His Tag) (Cat: 10001-H08H), the EC50 is 140-450 ng/mL (QC tested) .

Function

Asn971-Arg1023

Molecular Weight

The recombinant human Fc/EGF chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 290 amino acids and has a calculated molecular mass of 33 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhFc/EGF monomer is approximately 37 kDa due to the glycosylation.