EFNB2 Protein (C-human IgG1-Fc, Canine)

Product Specifications

Abbreviation

EFNB2

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

1. Measured by its binding ability in a functional ELISA. 2. Immobilized human EphB4-His (Cat:10235-H08H) at 10μg/mL (100μL/well) can bind canine EFNB2-Fc3. The EC50 of canine EFNB2-Fc3 is 25.8-60.2 ng/mL.

Function

Met1-Ala229

Molecular Weight

The recombinant canine EFNB2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 443 amino acids and has a predicted molecular mass of 49.2 kDa. The apparent molecular mass of the protein is approximately 58 and 35 kDa in SDS-PAGE under reducing conditions.