EFNB2 Protein (C-His, Rat)

Product Specifications

Abbreviation

EFNB2

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 99 % as determined by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA. 1. Immobilized rat EFNB2-His (Cat:80107-R08H) at 10 μg/mL (100 μl/well) can bind mouse EPHB4-Fc (Cat:50582-M02H), The EC50 of mouse EPHB4-Fc (Cat:50582-M02H) is 19.0-44.5 ng/mL. 2. Immobilized rat EFNB2-His (Cat:80107-R08H) at 10 μg/mL (100 μl/well) can bind human EphB4-Fc (Cat:10235-H02H), The EC50 of human EphB4-Fc (Cat:10235-H02H) is 10.0-23.5 ng/mL.

Function

Met1-Ala229

Molecular Weight

The recombinant rat EFNB2 comprises 212 amino acids and has a predicted molecular mass of 23.5 kDa. The apparent molecular mass of the protein is approximately 33-39 kDa in SDS-PAGE under reducing conditions due to glycosylation.