EFNB2 Protein (C-His, Canine)

Product Specifications

Abbreviation

EFNB2

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized canine EFNB2-His at 10 μg/ml (100 μl /well) can bind human EphB4-Fc (Cat:10235-H02H), The EC50 of human EphB4-Fc (Cat:10235-H02H) is 9-20 ng/ml.

Function

Met1-Ala229

Molecular Weight

The recombinant canine EFNB2 comprises 213 amino acids and has a predicted molecular mass of 23.6 kDa. The apparent molecular mass of the protein is approximately 33-38 kDa in SDS-PAGE under reducing conditions due to glycosylation.