EFNA3 Protein (C-human IgG1-Fc, Human)

1.Measured by its binding ability in a functional ELISA. Immobilized mouse EphA6 at 1 μg/ml (100 μl/well) can bind human EphrinA3 / Fc Chimera. The EC50 of human EphrinA3 is 299.2 ng/mL. 2.Loaded Recombinant Human Ephrin A3 Protein, hFc Tag (Cat. No. 10188-H02H) on proA Biosensor, can bind Recombinant Human EphA6 Protein, His Tag (Cat. No. 16039-H08H) with an affinity constant of 43.1 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Loaded Recombinant Human Ephrin A3 Protein, hFc Tag (Cat. No. 10188-H02H) on proA Biosensor, can bind Recombinant Cynomolgus EPHA5 Protein, His Tag (Cat. No. 91049-C08H) with an affinity constant of 78.2 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Loaded Recombinant Human Ephrin A3 Protein, hFc Tag (Cat. No. 10188-H02H) on ProA Biosensor, can bind Recombinant Human EPHA5 Protein, His Tag (Cat. No. 29795-H08H) with an affinity constant of 0.65 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human EphrinA3/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 429 amino acids and has a predicted molecular mass of 48 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEphrinA3/Fc monomer is approximately 60-65 kDa due to glycosylation.