DPP4 Protein (N-human IgG1-Fc, Human)

1. Measured by its ability to bind recombinant Cynomolgus CXCL12 in a functional ELISA. 2. Measured by its ability to bind recombinant Human SDF1b in a functional ELISA. 3. Loaded Fc-DPPIV (Cat: 10688-H01H) on ProA Biosensor, can bind MERS-CoV Spike/RBD Protein fragment (RBD, aa 367-606, His Tag) (Cat. 40071-V08B1) with an affinity constant of 1.04 nM as determined by Octet RED System. 4. Loaded Fc-DPPIV (Cat: 10688-H01H) on ProA Biosensor, can bind MERS-CoV Spike/S1 Protein (S1 Subunit, aa 1-725, His Tag) (Cat. 40069-V08H) with an affinity constant of 0.02 nM as determined by Octet RED System. 5.Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC) . The specific activity is > 2000 pmoles/min/μg (QC tested) .

The recombinant human Fc/DPPIV is a disulfide-linked homodimeric protein.The reduced monomer consists of 975 amino acids and has a predicted molecular mass of 112 kDa. As a result of glycosylation, the apparent molecular mass of rhFc/DPPIV monomer is approximately 120-130 kDa in SDS-PAGE under reducing conditions.