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DPP4 Protein (N-cleavage, Human)

Product Specifications

Abbreviation

DPP4

Synonyms

ADABP, ADCP2, CD26, DPPIV, TP103

Host

HEK 293

Tag

N-cleavage

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 70 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1. Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC) . The specific activity is > 2,500 pmoles/min/μg. 2. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 10688-HNCH) bound to Spike (HCoV-EMC/2012) (Cat: 40071-V31B) was 20 nM. 3. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 10688-HNCH) bound to Spike (HCoV-EMC/2012) (Cat: 40071-V05B) was 50 nM. 4. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 10688-HNCH) bound to Spike (HCoV-EMC/2012) (ECD, aa 1-1297) (Cat: 40069-V08B) was 33 nM. 5. Using the Octet RED System, the affinity constant (Kd) of human DPP4 (Cat: 10688-HNCH) bound to Spike-His (aa 1-760) (Cat: 40021-V08H) was 12 nM.

Function

Asn29-Pro766

Molecular Weight

The recombinant human DPPIV exists as the mature active form, and consists of 738 amino acids with a predicted molecular mass of 85.4 kDa. The apparent molecular mass of the glycosylated rhDPPIV is approximately 95 kDa in SDS-PAGE under reducing conditions.

Species

Human

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