DLL4 Protein (C-His, Human)

1.Immobilized Recombinant Human NOTCH1 Protein (Fc Tag) (Cat: 10954-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human DLL4 / Delta4 Protein (His Tag) (Cat: 10171-H08H), the EC50 is 100-300 ng/mL. 2.Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-10 μg/mL in the presence of 500 ng/mL recombinant human BMP2. 3.Loaded Recombinant Human NOTCH1 Protein, hFc Tag (Cat. No. 10954-H02H) on ProA Biosensor, can bind Recombinant Human DLL4 Protein, His Tag (Cat. No. 10171-H08H) with an affinity constant of 51.7nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Recombinant Human NOTCH1 Protein, hFc Tag (Cat. No. 10954-H02H) captured on Protein A chip can bind Recombinant Human DLL4 Protein, His Tag (Cat. No. 10171-H08H) with an affinity constant of 1.356 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The recombinant human DLL4 consists of 509 amino acids and predicts a molecular mass of 55.7 kDa. As a result of glycosylation, the apparent molecular mass of rhDLL4 is approximately 55-70 kDa band in SDS-PAGE under reducing conditions.