DKK1 Protein (C-human IgG1-Fc, Human)

1.Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.01-0.07μg/mL in the presence of 10 ng/mL of mouse Wnt3a. 2.Loaded Recombinant Human LRP-6 Protein, His & AVI Tag, Avi-tag Biotinylated (Cat. No. 11968-H49H-B) on SA Biosensor, can bind Recombinant Human DKK-1 Protein, hFc Tag (Cat. No. 10170-H02H) with an affinity constant of 54.3 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .