DKK1 Protein (C-His, Rhesus)

Product Specifications

Abbreviation

DKK1

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 2A6 mouse embryonal fibroblast cells . The ED50 for this effect is typically 0.5-3 μg/ml in the presence of 10 ng/mL of Wnt3a.

Function

Met2-His266,256Asn/Gln

Molecular Weight

The recombinant rhesus DKK1 comprises 246 amino acids with a predicted molecular mass of 27.2 kDa. As a result of glycosylation, the apparent molecular mass of rhesus DKK1 is approximately 38-43 kDa in SDS-PAGE under reducing conditions.