CTSS Protein (C-His, Human)

Product Specifications

Abbreviation

CTSS

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to cleave a fluorogenic peptide substrate, (7-methoxycoumarin-4-yl) acetyl-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys (2, 4-dinitrophenyl) -NH2 (R&D Systems, Catalog#ES002) . Cleavage of ES002 can be measured using excitation and emission wavelength at 320 nm and 405 nm, respectively. The specific activity is >300 pmoles/min/μg . (Activation description: The enzyme achieves its activity under acidic pH)

Function

Met1-Ile331

Molecular Weight

The secreted recombinant human CTSS consists of 326 amino acids with the predicted molecular mass of 37 kDa as estimated by SDS-PAGE under reducing conditions.