CTLA4 Protein (C-human IgG1-Fc, Mouse)

Product Specifications

Abbreviation

CTLA4

Synonyms

Cd152, Ctla-4, Ctla4, Ly-56

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.05-0.3μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80. 2.Immobilized Recombinant Mouse CD86 / B7-2 Protein (His Tag) (Cat: 50068-M08H) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse CTLA4 / CD152 Protein (ECD, Fc Tag) (Cat: 50503-M02H), the EC50 is 2.0-7.0 ng/mL.

Function

Met1-Phe162

Molecular Weight

The secreted recombinant mouse CTLA4/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 368 amino acids and has a predicted molecular mass of 41 kDa. As a result of glycosylation, the apparent molecular mass of rm CTLA4/Fc monomer is approximately 53 kDa in SDS-PAGE under reducing conditions.