CTLA4 Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

CTLA4

Synonyms

ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Loaded Recombinant Human CTLA4 / CD152 Protein (Fc Tag) (Cat: 11159-H02H6) on ProA Biosensor, can bind Recombinant Human CD80 / B7-1 Protein (His Tag) (Cat: 10698-H08H) with an affinity constant of 210 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2.Immobilized Recombinant Human CTLA4 / CD152 Protein (Fc Tag) (Cat: 11159-H02H6) at 2 μg/mL (100 μL/well) can bind Anti-CTLA4 (Research grade Ipilimumab biosimilar), the EC50 is 2-6 ng/mL (Routinely tested) . 3.Immobilized Recombinant Human CD86 / B7-2 Protein (His Tag) (Cat: 10699-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human CTLA4 / CD152 Protein (Fc Tag) (Cat: 11159-H02H6), the EC50 is 3.5-10.5 ng/mL (Routinely tested) . 4.Immobilized Recombinant Human CTLA4 / CD152 Protein (Fc Tag) (Cat: 11159-H02H6) at 10 μg/mL (100 μL/well) can bind Recombinant Human CD80 / B7-1 Protein (His Tag) (Cat: 10698-H08H), the EC50 is 0.2-0.8 μg/mL (QC tested) .

Function

Met1-Phe162

Molecular Weight

The recombinant human CTLA4 consists of 368 amino acids and predicts a molecular mass of 40.7 kDa. As a result of glycosylation, the apparent molecular mass of human CTLA4 is approximately 46-55 kDa in SDS-PAGE under reducing conditions

Species

Human