CTLA4 Protein (C-human IgG1-Fc & AVI, Human)

1.Immobilized Recombinant Human CD86 / B7-2 Protein (Fc Tag) (Cat:10699-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human CTLA-4 Protein (ECD, Fc & AVI Tag), Biotinylated (Cat:11159-H41H-B), the EC50 is 3.6-11.5 ng/mL (QC tested) . 2.Immobilized Anti-CTLA4 (Research grade Ipilimumab biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human CTLA-4 Protein (ECD, Fc & AVI Tag), Biotinylated (Cat: 11159-H41H-B), the EC50 is 0.4-1.2 ng/mL (Routinely tested) . 3.Immobilized Recombinant Human CD80 / B7-1 Protein (ECD, Fc Tag) (Cat: 10698-H02H) at 5 μg/mL (100 μL/well) can bind Recombinant Human CTLA-4 Protein (ECD, Fc & AVI Tag), Biotinylated (Cat: 11159-H41H-B), the EC50 is 4.4 ng/mL (Routinely tested) . 4.Immobilized Recombinant Cynomolgus / Rhesus CD86 / B7-2 Protein (His Tag) (Cat: 90270-C08H) at 5 μg/mL (100 μL/well) can bind Recombinant Human CTLA-4 Protein (ECD, Fc & AVI Tag), Biotinylated (Cat: 11159-H41H-B), the EC50 is 163.8 ng/mL (Routinely tested) . 5.Immobilized Recombinant Human CTLA-4 Protein (ECD, Fc & AVI Tag), Biotinylated (Cat: 11159-H41H-B) at 1 μg/mL (100 μL/well) on streptavidin precoated (5 μg/mL, 100 μL/well) plate can bind Anti-CTLA4 (Research Grade Ipilimumab Biosimilar), the EC50 is 1.8 ng/mL (Routinely tested) .

The recombinant Human CTLA4 consists of 386 amino acids and predicts a molecular mass of 42.89 kDa. It migrates as an approximately 53.61 kDa band in SDS-PAGE under reducing conditions.