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CTLA4 Protein (C-His, Cynomolgus, Rhesus)

Product Specifications

Abbreviation

CTLA4

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

1.Immobilized cynomolgus/rhesus CTLA4-His at 10 μg/ml (100 μl/well) can bind Cynomolgus CD80-Fc (Cat:90268-C02H), The EC50 of cynomolgus/rhesus CD80-Fc (Cat:90268-C02H) is 0.02-0.04 μg/ml (QC tested) . 2.Immobilized Anti-CTLA4 (Research grade Ipilimumab biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus / Rhesus CTLA4 / CD152 Protein (His Tag) (Cat: 90213-C08H), the EC50 is 0.8-2.4 ng/mL (Routinely tested) . 3.Immobilized Recombinant Cynomolgus / Rhesus CD86 / B7-2 Protein (Fc Tag) (Cat: 90270-C02H) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus / Rhesus CTLA4 / CD152 Protein (His Tag) (Cat: 90213-C08H), the EC50 is 15.4 ng/mL (Routinely tested) .

Function

Met1-Asp161

Molecular Weight

The recombinant cynomolgus / rhesus CTLA4 comprises 136 amino acids and has a calculated molecular mass of 14.9 KDa. The apparent molecular mass of it is approximately 24 and 21 KDa respectively in SDS-PAGE due to different glycosylation.

Species

Cynomolgus, Rhesus

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