CSF3 Protein (N-human IgG1-Fc, Human)

Product Specifications

Abbreviation

CSF3

Synonyms

C17orf33, CSF3OS, GCSF, MGC45931

Host

HEK 293

Tag

N-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 97 % as determined by SDS-PAGE

Bioactivity

1.Measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60. The ED50 for this effect is typically 0.2-0.8 ng/ml (Routinely tested) . 2.Immobilized Recombinant Human G-CSFR / CD114 Protein (His Tag) (Cat: 10218-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human G-CSF / CSF3 Protein (Fc Tag) (Cat: 10007-H01H), the EC50 is 5-15 ng/mL (QC tested) .

Function

Ala30-Pro204

Molecular Weight

The recombinant mature human GCSFb/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 412 amino acids and has a calculated molecular mass of 45.4 kDa. As a result of glycosylation, the rh GCSFb/Fc monomer migrates as an approximately 48 kDa protein in SDS-PAGE under reducing conditions.