CSF2 Protein (N-human IgG1-Fc, Human)

Product Specifications

Abbreviation

CSF2

Synonyms

CSF, GM-CSF, GMCSF

Host

HEK 293

Tag

N-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 97 % as determined by SDS-PAGE

Bioactivity

1.Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 1-5 ng/mL (Routinely tested) . 2.Immobilized Recombinant Human GM-CSF / CSF2 Protein (Fc Tag) (Cat: 10015-H01H) at 2 μg/mL (100 μL/well) can bind Recombinant Human CSF2RA / GM-CSFR / CD116 Protein (His Tag) (Cat: 10701-H08H), the EC50 is 15-45 ng/mL (QC tested) .

Function

Ala18-Glu144

Molecular Weight

The recombinant human GM-CSF/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 364 amino acids and has a predicted molecular mass of 41 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh GM-CSF/Fc monomer is approximately 48-55 kDa due to glycosylation.