CSF1R Protein (C-human IgG1-Fc, Human)

1.Measured by its ability to inhibit the human CSF-induced proliferation of M‐NFS‐60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 0.05-0.3μg/mL in the presence of 3 ng/ml Recombinant Human M-CSF (Routinely tested) . 2.Recombinant Human M-CSFR/CSF1R Protein, hFc Tag (Cat. No. 10161-H02H) captured on Protein A chip can bind Recombinant Human M-CSF/CSF1 Protein, His Tag (Cat. No. 11792-H08H) with an affinity constant of 0.216 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Loaded Recombinant Human M-CSF/CSF1 Protein, His Tag (Cat. No. 11792-H08H) on HIS1K Biosensor, can bind Recombinant Human M-CSFR/CSF1R Protein, hFc Tag (Cat. No. 10161-H02H) with an affinity constant of 4.350 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Loaded Biotinylated Recombinant Human M-CSF/CSF1 Protein, His & AVI Tag (Cat. No. 11792-H49H-B) on SA Biosensor, can bind Recombinant Human M-CSFR/CSF1R Protein, His Tag (Cat. No. 10161-H08H) with an affinity constant of 0.179 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 5.Immobilized Recombinant Human M-CSF / CSF-1 Protein (His Tag) (Cat: 11792-H08H) at 5 μg/mL (100 μL/well) can bind Recombinant Human CSF1R / MCSF Receptor / CD115 Protein (Fc Tag) (Cat: 10161-H02H), the EC50 is 5.0-15.5 ng/mL. 6.Loaded Recombinant Human CSF1R / MCSF Receptor / CD115 Protein (Fc Tag) (Cat. No. 10161-H02H) on ProA Biosensor, can bind Recombinant Human M-CSF / CSF-1 Protein (His Tag) (Cat. No. 11792-H08H) with an affinity constant of 0.248 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .