CSF1R Protein (C-human IgG1-Fc, Rat)

1. Immobilized rat CSF1R-Fc at 10 μg/ml (100 μl/well) can bind biotinylated human CSF1-His (Cat:11792-H08H), The EC50 of biotinylated human CSF1-His (Cat:11792-H08H) is 1.7-3.9 ng/ml. 2. Measured by its ability to inhibit mouse CSF induced proliferation of M‐NFS‐60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 50-250 ng/mL in the presence of 2.5 ng/mL Recombinant Mouse M-CSF.

The recombinant rat CSF1R/Fc is a disulfide-linked homodimer. The reduced monomer comprises 732 amino acids and has a predicted molecular mass of 82 kDa. The apparent molecular mass of the protein is approximately 106-116 kDa in SDS-PAGE under reducing conditions due to glycosylation.