CSF1 Protein (C-His-AVI, Human)

1.Immobilized Recombinant Human CSF1R / MCSF Receptor / CD115 Protein (His Tag) (Cat: 10161-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human M-CSF/CSF1 Protein (His & AVI Tag), Biotinylated (Cat: 11792-H49H-B), the EC50 is 1.4-4.2 ng/mL. 2.Loaded Biotinylated Recombinant Human M-CSF/CSF1 Protein, His & AVI Tag (Cat. No. 11792-H49H-B) on SA Biosensor, can bind Recombinant Human M-CSFR/CSF1R Protein, His Tag (Cat. No. 10161-H08H) with an affinity constant of 0.179 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Loaded Biotinylated Recombinant Human M-CSF/CSF1 Protein, His & AVI Tag (Cat. No. 11792-H49H-B) on SA Biosensor, can bind Recombinant Human M-CSFR/CSF1R Protein, hFc Tag (Cat. No. 10161-H02H) with an affinity constant of 9.870 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant Human CSF1 consisting of 249 amino acids and has a calculated molecular mass of 28.39 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 44.3 kDa protein in SDS-PAGE under reducing conditions.