CRISPR-Cas9 Protein (C-His, Streptococcus pyogenes)
Product Specifications
Abbreviation
CRISPR-Cas9
Host
Baculovirus-insect Cells
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Bioactivity
1. 1 μg = 5 U 1 U is defined as cutting 1 ug 1 KB dsDNA in 30 min at 37°C 2. CRISPR-Cas9 Protein (Cat#40572-A08B) achieves ≥ 90% substrate cleavage measured by an in vitro DNA cleavage assay.
Function
Met1-Asp1368
Molecular Weight
The recombinant s. pyogenes serotype M1 cas9 consists of 1407 amino acids and predicts a molecular mass of 163 kDa.
Species
Streptococcus pyogenes
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