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CRISPR-Cas9 Protein (C-His, Streptococcus pyogenes)

Product Specifications

Abbreviation

CRISPR-Cas9

Host

Baculovirus-insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1. 1 μg = 5 U 1 U is defined as cutting 1 ug 1 KB dsDNA in 30 min at 37°C 2. CRISPR-Cas9 Protein (Cat#40572-A08B) achieves ≥ 90% substrate cleavage measured by an in vitro DNA cleavage assay.

Function

Met1-Asp1368

Molecular Weight

The recombinant s. pyogenes serotype M1 cas9 consists of 1407 amino acids and predicts a molecular mass of 163 kDa.

Species

Streptococcus pyogenes

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