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CD86 Protein (C-His, Rat)

Product Specifications

Abbreviation

CD86

Synonyms

B7-2

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

1.Immobilized rat CD86 at 2 μg/ml (100 μl/well) can bind mouse CD28 with a linear range of 6.4-160 ng/ml (QC tested) . 2.Immobilized Recombinant Rat CTLA-4 / CD152 Protein (ECD, Fc Tag) (Cat: 81069-R02H) at 5 μg/mL (100 μL/well) can bind Recombinant Rat CD86 / B7-2 Protein (His Tag) (Cat: 80025-R08H), the EC50 is 292 ng/mL (Routinely tested) . 3.Immobilized Recombinant Mouse CTLA4 / CD152 Protein (ECD, Fc Tag) (Cat: 50503-M02H) at 5 μg/mL (100 μL/well) can bind Recombinant Rat CD86 / B7-2 Protein (His Tag) (Cat: 80025-R08H), the EC50 is 671.5 ng/mL (Routinely tested) .

Function

Met1-Ile250

Molecular Weight

The recombinant rat CD86 comprises 232 amino acids and predicts a molecular mass of 26.8 kDa. The apparent molecular mass of the rat CD86 is approximately 40-70 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Species

Rat

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