CD80 Protein (C-human IgG1-Fc, Human)

1.Loaded Recombinant Human B7-1/CD80 Protein, hFc Tag (Cat. No. 10698-H02H) on ProA Biosensor, can bind Recombinant Human CTLA-4 Protein (Cat. No. 11159-HNAH) with an affinity constant of 2.09 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2.Loaded Recombinant Human B7-1/CD80 Protein, hFc Tag (Cat. No. 10698-H02H) on proA Biosensor, can bind Recombinant Human, Cynomolgus, Rhesus CD28 Protein, His Tag (Cat. No. 90182-C08H) with an affinity constant of 0.895 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Immobilized Recombinant Human CD80 / B7-1 Protein (ECD, Fc Tag) (Cat: 10698-H02H) at 5 μg/mL (100 μL/well) can bind Recombinant Human CTLA-4 Protein (ECD, Fc & AVI Tag), Biotinylated (Cat: 11159-H41H-B), the EC50 is 2.3 ng/mL (Routinely tested) . 4.Immobilized Recombinant Human CD80 / B7-1 Protein (ECD, Fc Tag) (Cat: 10698-H02H) at 5 μg/mL (100 μL/well) can bind Recombinant Human PD-L1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10084-H41H-B), the EC50 is 243 ng/mL (Routinely tested) .

The recombinant human CD80 consists of 446 amino acids and predicts a molecular mass of 50.6 kDa. As a result of glycosylation, it migrates as an approximately 65.8 kDa band in SDS-PAGE under reducing conditions.