CD80 Protein (C-human IgG1-Fc & AVI, Human)

1.Immobilized Recombinant Human CTLA4 / CD152 Protein (Fc Tag) (Cat: 11159-H02H6) at 2 μg/mL (100 μl/well) can bind Recombinant Human B7-1/CD80 Protein (Fc & AVI Tag), Biotinylated (Cat: 10698-H41H-B), the EC50 is 0.7-2.1 ng/mL (QC tested) . 2.Immobilized Recombinant Human PD-L1/B7-H1 Protein (ECD, hFc Tag), HPLC-verified (Cat: 10084-H02H) at 5 μg/mL (100 μL/well) can bind Recombinant Human B7-1/CD80 Protein (hFc & AVI Tag), Biotinylated, HPLC-verified (Cat: 10698-H41H-B) the EC50 is 571 ng/mL (Routinely tested) . 3.Serial dilutions of anti-CTLA4 (Research Grade Ipilimumab biosimilar) were added into Recombinant Human CTLA4 / CD152 Protein (Fc Tag) (Cat: 11159-H02H6) : Recombinant Human B7-1/CD80 Protein (Fc & AVI Tag), Biotinylated (Cat: 10698-H41H-B) binding reactions. The half maximal inhibitory concentration (IC50) is 340.9 ng/mL (Routinely tested) .

The recombinant Human CD80 consisting of 461 amino acids and has a calculated molecular mass of 52.4 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 73.6 kDa protein in SDS-PAGE under reducing conditions.