CD58 Protein (C-human IgG1-Fc, Human)

1. Measured by its binding ability in a functional ELISA. Immobilized human CD2-His (Cat:10982-H08H) at 10 μg/ml (100 μl/well) can bind human CD58-Fc, The EC50 of human CD58-Fc is 0.04-0.1 μg/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CD2-His (Cat:90300-C08H) at 10 μg/ml (100 μl/well) can bind human CD58-Fc, The EC50 of human CD58-Fc is 0.04-0.10 μg/ml. 3.Loaded Recombinant Human CD58/LFA-3 Protein, hFc Tag (Cat. No. 12409-H02H) on ProA Biosensor, can bind Recombinant Human CD2 Protein, His Tag (Cat. No. 10982-H08H) with an affinity constant of 1.36 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human CD58/Fc is a disulfide-linked homodimer. The reduced monomer comprises 428 amino acids and has a predicted molecular mass of 48.5 kDa. The apparent molecular mass of the protein is approximately 68 kDa in SDS-PAGE under reducing conditions.