CD47 Protein (C-His, Rat)

Product Specifications

Abbreviation

CD47

Synonyms

Cd47, Itgp

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Rat CD47-His at 10 μg/ml (100 μl/well) can bind mouse SIRPA-Fc (Cat:50956-M02H), The EC50 of mouse SIRPA-Fc (Cat:50956-M02H) is 0.6-1.4 μg/ml.

Function

Met1-Lys140

Molecular Weight

The recombinant rat CD47 comprises 133 amino acids and predicts a molecular mass of 15.2 kDa. The apparent molecular mass of the recombinant protein is approximately 27-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.