CD47 Protein (C-His, Cynomolgus, Rhesus)

Product Specifications

Abbreviation

CD47

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Recombinant Human SIRP alpha / CD172a Protein (Fc Tag) (Cat: 11612-H38H) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus / Rhesus CD47 Protein (His Tag) (Cat: 90869-C08H), the EC50 is 60-200 ng/mL (QC tested) . 2.Immobilized Anti-CD47 Antibody, Human IgG4 (Magrolimab) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus / Rhesus CD47 Protein (His Tag) (Cat: 90869-C08H), the EC50 is 0.5-4 ng/mL (Routinely tested) . 3.Loaded Magrolimab on AHC Biosensor, can bind Recombinant Cynomolgus, Rhesus CD47 Protein, His Tag (Cat. No. 90869-C08H) with an affinity constant of 11.8 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Magrolimab captured on Protein A chip can bind Recombinant Cynomolgus, Rhesus CD47 Protein, His Tag (Cat. No. 90869-C08H) with an affinity constant of 8.435 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .