CD40LG Protein (N-human IgG1-Fc, Mouse)
Product Specifications
Abbreviation
CD40LG
Synonyms
CD154, CD40-L, Cd40l, Cd40lg, gp39, HIGM1, IGM, IMD3, Ly-62, Ly62, T-BAM, Tnfsf5, Tnlg8b, TRAP
Host
HEK 293
Tag
N-human IgG1-Fc
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
≥ 80 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
Bioactivity
1.Measured by its binding ability in a functional ELISA. Immobilized Mouse CD40 His (Cat:50324-M08H) at 2 μg/ml (100 μl/well) can bind Mouse CD40 Ligand hFc (Cat:50327-M01H), the EC50 of Mouse CD40 Ligand hFc is 60-300 ng/mL. 2.Loaded Recombinant Mouse CD40 Protein, His Tag (Cat.No.50324-M08H) on His1K Biosensor, can bind Recombinant Mouse CD40 Ligand Protein, hFc Tag (Cat.No.50327-M01H) with an affinity constant of 87.2nM as determined in BLI assay (Sartorius Octet RH16) (Routinely tested) .
Function
Gly115-Leu260
Molecular Weight
The recombinant mouse CD40LG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 406 amino acids and has a calculated molecular mass of 44.4 kDa. The apparent molecular mass of the monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.
Species
Mouse
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