CD40LG Protein (C-His, Human)

1.Immobilized Human CD40 Ligand (His Tag) (Cat:10239-H08E) at 2 μg/mL (100 μL/well) can bind Human CD40 (ECD, hFc Tag) (Cat:10774-H02H), the EC50 of Human CD40 (ECD, hFc Tag) (Cat:10774-H02H) is 60-600 ng/mL. 2.Loaded Recombinant Human CD40/TNFRSF5 Protein, hFc Tag (Cat. No. 10774-H02H) can bind Recombinant Human CD40 Ligand/TNFSF5 Protein, His Tag (Cat. No. 10239-H08E) with an affinity constant of 3.06 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human CD40L consists of 161 amino acids and has a calculated molecular mass of 17.7 kDa. It migrates as an 18 kDa band in SDS-PAGE under reducing conditions.