CD40 Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

CD40

Synonyms

Bp50, CDW40, p50, TNFRSF5

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Recombinant Human CD40L / CD154 / TNFSF5 Protein (His Tag) (Cat: 10239-H08E) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD40 / TNFRSF5 Protein (ECD, Fc Tag) (Cat: 10774-H02H), the EC50 is 10-30 ng/mL. 2.Loaded Recombinant Human CD40/TNFRSF5 Protein, hFc Tag (Cat. No. 10774-H02H) can bind Recombinant Human CD40 Ligand/TNFSF5 Protein, His Tag (Cat. No. 10239-H08E) with an affinity constant of 3.06 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Arg193

Molecular Weight

The recombinant human CD40 consists 414 amino acids and predicts a molecular mass of 46.2 kDa. As a result of glycosylation, the apparent molecular mass of human CD40 is approximately 54.1 kDa in SDS-PAGE under reducing conditions.