CD4 Protein (C-His, Ferret)

Product Specifications

Abbreviation

CD4

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 97 % as determined by SDS-PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.When 5 x 10E4 cells/well are added to CD4-coated plates (1.25μg/mL and 100μL/well), approximately >50% will adhere specifically after 30 minutes at 37°C.

Function

Met1-Leu401

Molecular Weight

The recombinant Ferret CD4 consists of 386 amino acids and has a calculated molecular mass of 42.7 kDa. The apparent molecular mass of the recombinant protein is approximately 52 kDa in SDS-PAGE under reducing conditions due to glycosylation.