CD3E & CD3G Protein (C-His-AVI, Human)

1.Immobilized Recombinant Human CD3E & CD3G Heterodimer Protein (His & AVI Tag), Biotinylated (Cat: CT123-H49H-B) at 1 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-CD3 (Research Grade OKT3 Biosimilar), the EC50 is 1.5-4.5 ng/mL (QC tested) . 2.Immobilized Recombinant Human CD3E & CD3G Heterodimer Protein (His & AVI Tag), Biotinylated (Cat: CT123-H49H-B) at 1 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-CD3 (Research Grade Clone SP34), the EC50 is 4-12 ng/mL (Routinely tested) . 3.Loaded Biotinylated Recombinant Human CD3E & CD3G Heterodimer Protein, His & AVI Tag (Cat. No. CT123-H49H-B) on HIS1K Biosensor, can bind Anti-CD3 (Research Grade OKT3 Biosimilar) with an affinity constant of 16.920 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Immobilized Anti-CD3 (Research Grade Clone SP34) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD3E & CD3G Heterodimer Protein (His & AVI Tag), Biotinylated (Cat: CT123-H49H-B), the EC50 is 23.5 ng/mL (Routinely tested) .

It migrates as an approximately 29.8、24 and 21.8 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 40.9 kDa verified by SEC-MALS (Routinely tested) .