CD3D & CD3E Protein (C-human IgG1-Fc & Flag & C-human IgG1-Fc & His, Human)

Product Specifications

Abbreviation

CD3D & CD3E

Host

HEK 293

Tag

C-human IgG1-Fc & Flag & C-human IgG1-Fc & His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

Label muromonab (mOKT3) -Anti-CD3 antibody, Mouse IgG2a with biotin. Immobilized Recombinant Human CD3D & CD3E Heterodimer Protein (Fc - FLAG & Fc - His Tag) (Cat: CT026-H2303H1) at 1 μg/mL (100 μL/well) can bind biotinylated muromonab (mOKT3) -Anti-CD3 antibody, Mouse IgG2a, the EC50 is 5-15 ng/mL (QC tested) .

Function

Met1-Ala105 & Met1-Asp126

Molecular Weight

It migrates as an approximately 49.7 and 44 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 85.4 kDa verified by SEC-MALS (Routinely tested) .

Species

Human