CD28 Protein (C-mouse IgG2a-Fc, Human, Cynomolgus, Rhesus)

Product Specifications

Abbreviation

CD28

Host

HEK 293

Tag

C-mouse IgG2a-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

Loaded Recombinant Human, Cynomolgus, Rhesus CD28 Protein, mFc Tag (Cat. No. 11524-H38H) on proA Biosensor, can bind Recombinant Human B7-1/CD80 Protein, His Tag (Cat. No. 10698-H08H) with an affinity constant of 24.200 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Pro152

Molecular Weight

The recombinant Human / Cynomolgus / Rhesus CD28 consists of 367 amino acids and predicts a molecular mass of 41.52 kDa. It migrates as an approximately 58.66 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 118.5 kDa verified by SEC-MALS (Routinely tested) .